LncRNA简介

 

LncRNA (Long noncoding RNAs, LncRNAs) ZHANGLIANFEIBIANMARNA， QICHUBEIRENWEISHIJIYINZUZHUANLUDE“”，SHIRNAJUHEMEIIIZHUANLUDEFUCHANWU，BUJUYOUSHENGWUXUEGONGNENG。RANER，JINNIANLAIDEYANJIUBIAOMING，LncRNACANYULEXRANSETICHENMO（TUA），YIJIRANSEZHIXIUSHI，（TUB），ZHUANLUGANRAO，HENEIYUNSHUDENGDUOZHONGZHONGYAODEDIAOKONGGUOCHENG，LncRNADEZHEIXIEDIAOKONGZUOYONGYEKAISHIYINQIRENMENGUANGFANDEGUANZHU。

 

BURUDONGWUJIYINZUXULIEZHONG4%~9%DEXULIECHANSHENGDESHILncRNA（XIANGYINGDEDANBAIBIANMARNADEBILISHI1%），SUIRANJINNIANLAIGUANYULncRNADEYANJIUJINZHANXUNMENG，DANSHIJUEDABUFENLncRNADEGONGNENGRENGBUQINGCHU，SUIZHEYANJIUDETUIJIN，GELEI LncRNA DEDALIANGFAXIAN，LncRNA DEYANJIUJIANGSHI RNA JIYINZUYANJIUFEICHANGXIYINRENDEYIGEFANGXIANG，SHIRENMENZHUJIANRENSHIDAOJIYINZUCUNZAIRENLEIZHIZHISHENSHAODE“”。

 

 

TUA：LncRNACANYUXRANSETIDECHENMO；    TUB：LncRNACANYUJIYINDEZHUANLUJIHUO。

 

 

服务介绍

 

HANHENGSHENGWUDELncRNAZHUANJIATUANDUIKEYIWEININTIGONGCONGLncRNAFENXIJIBAJIYINYUCE，LncRNADEGONGNENGTANSUOYIJIGENGSHENCENGCIDEBADIANXUNZHAOHEBIAODADIAOKONGJIZHITANSUODENGQUANFANGWEIDEFUWU。

 

 

1、LncRNA与microRNA相关性分析及预测

 

 

A、LncRNA-mRNA/microRNAXIANGGUANXINGFENXI：GENJUSHEDINGDEYUZHISHAIXUANLncRNAYUmRNA/microRNAGUANXIDUI，GOUJIANLncRNA-mRNAGONGBIAODAWANGLUO。

 

B、LncRNABAJIYINYUCE：JIYULncRNAYUmRNA/miRNABIAODAXIANGGUANXINGYIJILncRNAYUmRNA/microRNAJIYINZUWEIZHIJINLINGUANXI，DEDAOLncRNADEQIANZAIBABIAOJIYIN。YIJITONGGUOWANGLUOSHUJUKUmiR CODE、Cat RAPIDDENGJINXINGYUCELncRNAKENENGDIAOKONGDEmiRNAHEJIYIN。（GEXINGHUADINGZHI）

 

 

TU：miRcodeSHUJUKU

 

TU：CatRAPIDSHUJUKU

 

 

C、LncRNAGONGNENGFENXI：DUIYUYIZHIGONGNENGDELncRNA，WOMENDUILncRNAJINXINGGONGNENGBIAOZHU。DUIYUWEIZHIGONGNENGDELncRNA，WOMENYUCEQIGONGNENG，BINGJINXINGGONGNENGFUJIFENXI。（GEXINGHUADINGZHI）

 

 

2、LncRNA的功能研究

 

A、LncRNADEBIAODABIANHUA-HANHENGSHENGWUDUYOUGAOLINGMINDULncRNAJIANCEJISHU（LncPrimerTM）

TONGGUOHANHENGSHENGWUDUYOUGAOLINGMINDULncRNAJIANCEYINWUKU（LncPrimerTM），LIYONGQPCR、FISHJISHU，ZAIGEZHONGXIBAO、DONGWUMOXINGZHONG，JIANCELncRNAZAIXIBAOHEZUZHISHUIPINGDEFENBUHEBIANHUA。

 

B、Gain of  Function（TINEIHETIWAI）

LIYONGHANHENGSHENGWUHanbioTMXILIEGAOXIAOBIAODABINGDUZAITI（MANBINGDU、XIANBINGDUDENG）QUANZHANGGUOBIAODALncRNA

 

C、Loss of  Function（TINEIHETIWAI）

◆ HECHENGPIANDUANsiRNA；

◆ HANHENGSHENGWUHanbioTMXILIEGAOXIAOBIAODABINGDUZAITI（MANBINGDU、XIANBINGDUDENG）BIAODAshRNA；

◆ ASO（antisenseoligonucleotide，FANYIGUAHEGANSUAN），ZHUYAOZHENDUIXIBAOHENEIDEFANYIRNAQIZUOYONG。

 

D、LncRNACHENMOYUDINGWEIFENXI(Combined knockdown and localization analysis of noncoding RNAs, c-KLAN)JISHU 

 

 

TU：c-KLANJISHU

 

 

3、 差异性的LncRNA的筛选

 

A、SHENGWUXINXIXUEYUCE

 

GENJUKEHUTIGONGDEZHUANLUZUXINXI、JIYINTEZHENG，TONGGUOYUSHUJUKU（agilent_ncRNA、LncRNAdb、Gencode V13、H-invDB、UCSC_lincRNAsTranscriptsDENG）BIJIAO，QUCHUYIZHIKENENGDANBAIBIANMAZHUANLUBEN、tRNA、rRNA、repeatDENGYUCEKENENGDELncRNA

 

B、GAOTONGLIANGDEBIAODAPUXINPIAN

 

 

C、XINYIDAIDECEXU

 

JUYOUXIANDAIDEDNACEXUJISHUDEKUAISUCEXUSUDUYIJINGYOUZHUYUDADAOCEXUWANZHENGDEDNAXULIE。TONGGUONIZHUANLUFA，JIANGLncRNANIZHUANLUCHENGcDNA，QUEDINGMOUYIDUANXULIE，ZAITONGGUORACEFANGFA，QUEDINGYIZHIXULIEDELIANGDUANDEXULIE。

 

 

4、生物信息学分析

 

GENJUGAOTONGLIANGXINPIANSHUJU，JINGGUODAXINGFUWUQICHULI，JINXINGYIXIAJIXIANGZHUANYEDESHENGWUXINXIXUEFENXI：

 

 

 

A、XINPIANSHUJUYUCHULI：DUISHIYANSHUJUZHILIANGPINGGU，YUCHULIJIJUNYIHUACHULI。

 

B、CHAYILncRNAHEmRNA DESHAIXUAN：

             

 

TU：CHAYILncRNAHEmRNA DESHAIXUAN

 

C、JULEIFENXI：ZHENDUIXINPIANJIEGUOJINXINGYANGBENJICHAYIBIAODALncRNAHEmRNADEJULEI，XUNZHAOSHUYUTONGYIBIAODAQUSHIDEJIYINHUOYANGBEN。

 

 

TU：JULEIFENXI

 

 

D、GONGNENGFUJIFENXI：CHAYIJIYIN，YINGYONGSHUJUKUJINXINGGONGNENGFUJIFENXI，WAJUEJUYOUTONGJIXUEYIYIDECHAYIBIAODAJIYINDEGONGNENGLEIBIE。

 

 

TU：GONGNENGFUJIFENXIJULEIFENXI

 

 

5、LncRNA的调控机制研究 

 

A、BIAOGUANYICHUANYANJIU-DNAJIAJIHUA

LIYONGMSP、BSPJISHU，YANJIULncRNADUIDNAJIAJIHUADEDIAOKONG。

 

B、BIAOGUANYICHUANYANJIU-RANSEZHIZHONGSU

LIYONGChIPJISHU，YANJIULncRNADUIRANSEZHIZHONGSUDEDIAOKONG。

 

C、ZHUANLUDIAOKONGYANJIU

TONGGUOEMSA、RIP（XIATUSUOSHI）、ChIRP、Co-IPDENGJISHU，YANJIULncRNAYUZHUANLUYINZIJIANDEXIANGHUZUOYONG。

 

 

TU：RIPJISHULIUCHENG

 

 

技术优势

 

1.HANHENGSHENGWUYONGYOUYOURUOGANLncRNAYANJIUZHUANJIAZUCHENGDEZHUANYELncRNAYANJIUTUANDUI，JISHUSHUIPINGZHUOYUE，ZHUANZHUYULncRNAZHENGTIFUWU，KEWEININTIGONGYITIHUADELncRNAXITONGYANJIUFANGAN；

 

2.HANHENGSHENGWUYICHENGGONGWANCHENGXUDUOLncRNAXIANGMU，YONGYOUCHENGSHUDELncRNAXIANGGUANJINGYAN；

 

3.如需要详细的LncRNA课题/实验指导，欢迎登陆汉恒官网deborahseban.com与汉恒生物的LncRNA专家交流探讨更多细胞功能学研究领域。直击以下链接通过QQ与我们的技术工程师一对一交流！

 

 

 

 

参考文献

 

[1]Telomere and Subtelomere R-loops and Antigenic Variation in Trypanosomes

（2019NIANFABIAOYUJournal of Molecular Biology.  ZUOZHE：Li B）

 

[2]lncRNA GHET1 promotes cervical cancer progression through regulating AKT/mTOR and Wnt/β-catenin signaling pathways

（2019NIANFABIAOYUBioscience Reports，ZUOZHE：Shi H）

 

[3] LncRNA HOXA11-AS regulates calcium oxalate crystal-induced renal inflammation via miR-124-3p/MCP-1

(2019NIANFABIAOYUJ Cell Mol Med，ZUOZHE：Chen W)

 

[4] Targeting the microglial NLRP3 inflammasome and its role in Parkinson's disease

（2019NIANFABIAOYUMovement Disorders，ZUOZHELu M）

 

[5] GLUT-1 participates in the promotion of LncRNA CASC9 in proliferation and metastasis of laryngeal carcinoma cells.

（2019NIANFABIAOYUGene ，ZUOZHE Xi J）

 

[6] Long noncoding RNA MIAT: A potential role in the diagnosis and mediation of acute myocardial infarction

（2019NIANFABIAOYUMolecular Medical Reports，ZUOZHE：Rao F）

 

[7] Long Noncoding RNAs and Repetitive Elements: Junk or Intimate Evolutionary Partners

（2019NIANFABIAOYUGene，ZUOZHEZhang Z）

 

[8]  LncRNA NKILA integrates RXFP1/AKT and NF-κB signalling to regulate osteogenesis of mesenchymal stem cells

（2019NIANFABIAOYUJ Cell Mol Med，ZUOZHEFan Y)