LncRNA研究

 

LncRNA简介

 

LncRNA (Long noncoding RNAs, LncRNAs) ZHANGLIANFEIBIANMARNA, QICHUBEIRENWEISHIJIYINZUZHUANLUDE“”,SHIRNAJUHEMEIIIZHUANLUDEFUCHANWU,BUJUYOUSHENGWUXUEGONGNENG。RANER,JINNIANLAIDEYANJIUBIAOMING,LncRNACANYULEXRANSETICHENMO(TUA),YIJIRANSEZHIXIUSHI,(TUB),ZHUANLUGANRAO,HENEIYUNSHUDENGDUOZHONGZHONGYAODEDIAOKONGGUOCHENG,LncRNADEZHEIXIEDIAOKONGZUOYONGYEKAISHIYINQIRENMENGUANGFANDEGUANZHU。

 

BURUDONGWUJIYINZUXULIEZHONG4%~9%DEXULIECHANSHENGDESHILncRNA(XIANGYINGDEDANBAIBIANMARNADEBILISHI1%),SUIRANJINNIANLAIGUANYULncRNADEYANJIUJINZHANXUNMENG,DANSHIJUEDABUFENLncRNADEGONGNENGRENGBUQINGCHU,SUIZHEYANJIUDETUIJIN,GELEI LncRNA DEDALIANGFAXIAN,LncRNA DEYANJIUJIANGSHI RNA JIYINZUYANJIUFEICHANGXIYINRENDEYIGEFANGXIANG,SHIRENMENZHUJIANRENSHIDAOJIYINZUCUNZAIRENLEIZHIZHISHENSHAODE“”。

 

 

TUA:LncRNACANYUXRANSETIDECHENMO;    TUB:LncRNACANYUJIYINDEZHUANLUJIHUO。

 

 

服务介绍

 

HANHENGSHENGWUDELncRNAZHUANJIATUANDUIKEYIWEININTIGONGCONGLncRNAFENXIJIBAJIYINYUCE,LncRNADEGONGNENGTANSUOYIJIGENGSHENCENGCIDEBADIANXUNZHAOHEBIAODADIAOKONGJIZHITANSUODENGQUANFANGWEIDEFUWU。

 

 

1、LncRNA与microRNA相关性分析及预测

 

 

A、LncRNA-mRNA/microRNAXIANGGUANXINGFENXI:GENJUSHEDINGDEYUZHISHAIXUANLncRNAYUmRNA/microRNAGUANXIDUI,GOUJIANLncRNA-mRNAGONGBIAODAWANGLUO。

 

B、LncRNABAJIYINYUCE:JIYULncRNAYUmRNA/miRNABIAODAXIANGGUANXINGYIJILncRNAYUmRNA/microRNAJIYINZUWEIZHIJINLINGUANXI,DEDAOLncRNADEQIANZAIBABIAOJIYIN。YIJITONGGUOWANGLUOSHUJUKUmiR CODE、Cat RAPIDDENGJINXINGYUCELncRNAKENENGDIAOKONGDEmiRNAHEJIYIN。(GEXINGHUADINGZHI)

 

 

TU:miRcodeSHUJUKU

 

TU:CatRAPIDSHUJUKU

 

 

C、LncRNAGONGNENGFENXI:DUIYUYIZHIGONGNENGDELncRNA,WOMENDUILncRNAJINXINGGONGNENGBIAOZHU。DUIYUWEIZHIGONGNENGDELncRNA,WOMENYUCEQIGONGNENG,BINGJINXINGGONGNENGFUJIFENXI。(GEXINGHUADINGZHI)

 

 

2、LncRNA的功能研究

 

A、LncRNADEBIAODABIANHUA-HANHENGSHENGWUDUYOUGAOLINGMINDULncRNAJIANCEJISHU(LncPrimerTM)

TONGGUOHANHENGSHENGWUDUYOUGAOLINGMINDULncRNAJIANCEYINWUKU(LncPrimerTM),LIYONGQPCR、FISHJISHU,ZAIGEZHONGXIBAO、DONGWUMOXINGZHONG,JIANCELncRNAZAIXIBAOHEZUZHISHUIPINGDEFENBUHEBIANHUA。

 

B、Gain of  Function(TINEIHETIWAI)

LIYONGHANHENGSHENGWUHanbioTMXILIEGAOXIAOBIAODABINGDUZAITI(MANBINGDU、XIANBINGDUDENG)QUANZHANGGUOBIAODALncRNA

 

C、Loss of  Function(TINEIHETIWAI)

◆ HECHENGPIANDUANsiRNA;

◆ HANHENGSHENGWUHanbioTMXILIEGAOXIAOBIAODABINGDUZAITI(MANBINGDU、XIANBINGDUDENG)BIAODAshRNA;

◆ ASO(antisenseoligonucleotide,FANYIGUAHEGANSUAN),ZHUYAOZHENDUIXIBAOHENEIDEFANYIRNAQIZUOYONG。

 

D、LncRNACHENMOYUDINGWEIFENXI(Combined knockdown and localization analysis of noncoding RNAs, c-KLAN)JISHU 

 

 

TU:c-KLANJISHU

 

 

3、 差异性的LncRNA的筛选

 

A、SHENGWUXINXIXUEYUCE

 

GENJUKEHUTIGONGDEZHUANLUZUXINXI、JIYINTEZHENG,TONGGUOYUSHUJUKU(agilent_ncRNA、LncRNAdb、Gencode V13、H-invDB、UCSC_lincRNAsTranscriptsDENG)BIJIAO,QUCHUYIZHIKENENGDANBAIBIANMAZHUANLUBEN、tRNA、rRNA、repeatDENGYUCEKENENGDELncRNA

 

B、GAOTONGLIANGDEBIAODAPUXINPIAN

 

 

C、XINYIDAIDECEXU

 

JUYOUXIANDAIDEDNACEXUJISHUDEKUAISUCEXUSUDUYIJINGYOUZHUYUDADAOCEXUWANZHENGDEDNAXULIE。TONGGUONIZHUANLUFA,JIANGLncRNANIZHUANLUCHENGcDNA,QUEDINGMOUYIDUANXULIE,ZAITONGGUORACEFANGFA,QUEDINGYIZHIXULIEDELIANGDUANDEXULIE。

 

 

4、生物信息学分析

 

GENJUGAOTONGLIANGXINPIANSHUJU,JINGGUODAXINGFUWUQICHULI,JINXINGYIXIAJIXIANGZHUANYEDESHENGWUXINXIXUEFENXI:

 

 

 

A、XINPIANSHUJUYUCHULI:DUISHIYANSHUJUZHILIANGPINGGU,YUCHULIJIJUNYIHUACHULI。

 

B、CHAYILncRNAHEmRNA DESHAIXUAN:

             

 

TU:CHAYILncRNAHEmRNA DESHAIXUAN

 

C、JULEIFENXI:ZHENDUIXINPIANJIEGUOJINXINGYANGBENJICHAYIBIAODALncRNAHEmRNADEJULEI,XUNZHAOSHUYUTONGYIBIAODAQUSHIDEJIYINHUOYANGBEN。

 

 

TU:JULEIFENXI

 

 

D、GONGNENGFUJIFENXI:CHAYIJIYIN,YINGYONGSHUJUKUJINXINGGONGNENGFUJIFENXI,WAJUEJUYOUTONGJIXUEYIYIDECHAYIBIAODAJIYINDEGONGNENGLEIBIE。

 

 

TU:GONGNENGFUJIFENXIJULEIFENXI

 

 

5、LncRNA的调控机制研究 

 

A、BIAOGUANYICHUANYANJIU-DNAJIAJIHUA

LIYONGMSP、BSPJISHU,YANJIULncRNADUIDNAJIAJIHUADEDIAOKONG。

 

B、BIAOGUANYICHUANYANJIU-RANSEZHIZHONGSU

LIYONGChIPJISHU,YANJIULncRNADUIRANSEZHIZHONGSUDEDIAOKONG。

 

C、ZHUANLUDIAOKONGYANJIU

TONGGUOEMSA、RIP(XIATUSUOSHI)、ChIRP、Co-IPDENGJISHU,YANJIULncRNAYUZHUANLUYINZIJIANDEXIANGHUZUOYONG。

 

 

TU:RIPJISHULIUCHENG

 

 

技术优势

 

1.HANHENGSHENGWUYONGYOUYOURUOGANLncRNAYANJIUZHUANJIAZUCHENGDEZHUANYELncRNAYANJIUTUANDUI,JISHUSHUIPINGZHUOYUE,ZHUANZHUYULncRNAZHENGTIFUWU,KEWEININTIGONGYITIHUADELncRNAXITONGYANJIUFANGAN;

 

2.HANHENGSHENGWUYICHENGGONGWANCHENGXUDUOLncRNAXIANGMU,YONGYOUCHENGSHUDELncRNAXIANGGUANJINGYAN;

 

3.如需要详细的LncRNA课题/实验指导,欢迎登陆汉恒官网deborahseban.com与汉恒生物的LncRNA专家交流探讨更多细胞功能学研究领域。直击以下链接通过QQ与我们的技术工程师一对一交流!

 

 

 

 

参考文献

 

[1]Telomere and Subtelomere R-loops and Antigenic Variation in Trypanosomes

(2019NIANFABIAOYUJournal of Molecular Biology.  ZUOZHE:Li B)

 

[2]lncRNA GHET1 promotes cervical cancer progression through regulating AKT/mTOR and Wnt/β-catenin signaling pathways

(2019NIANFABIAOYUBioscience Reports,ZUOZHE:Shi H)

 

[3] LncRNA HOXA11-AS regulates calcium oxalate crystal-induced renal inflammation via miR-124-3p/MCP-1

(2019NIANFABIAOYUJ Cell Mol Med,ZUOZHE:Chen W)

 

[4] Targeting the microglial NLRP3 inflammasome and its role in Parkinson's disease

(2019NIANFABIAOYUMovement Disorders,ZUOZHELu M)

 

[5] GLUT-1 participates in the promotion of LncRNA CASC9 in proliferation and metastasis of laryngeal carcinoma cells.

(2019NIANFABIAOYUGene ,ZUOZHE Xi J)

 

[6] Long noncoding RNA MIAT: A potential role in the diagnosis and mediation of acute myocardial infarction

(2019NIANFABIAOYUMolecular Medical Reports,ZUOZHE:Rao F)

 

[7] Long Noncoding RNAs and Repetitive Elements: Junk or Intimate Evolutionary Partners

(2019NIANFABIAOYUGene,ZUOZHEZhang Z)

 

[8]  LncRNA NKILA integrates RXFP1/AKT and NF-κB signalling to regulate osteogenesis of mesenchymal stem cells

(2019NIANFABIAOYUJ Cell Mol Med,ZUOZHEFan Y)

 

 

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