CRISPR/Cas9基因敲除技术服务

 

汉恒生物CRISPR/Cas9 亚博app官网及服务

● DUOZHONGBIAOQIANDECRISPR/Cas9 ZHILI

● CRISPR/Cas9MANBINGDU、XIANBINGDUJIXIANXIANGGUANBINGDU(AAV)BAOZHUANG

● CRISPR/Cas9DANKELONGQIAOCHUXIBAOZHU


 

CRISPR/Cas9 基因敲除原理介绍

CRISPR (clustered, regularly interspaced, short palindromic repeats)SHIYIZHONGLAIZIXIJUNJIANGJIERUQINDEBINGDU DNA HUOQITAWAIYUAN DNA DEMIANYIJIZHI。ZAIXIJUNJIGUXIJUNZHONG,CRISPRXITONGGONGFENCHENG3LEI,QIZHONGⅠLEIHEⅢLEIXUYAODUOZHONGCRISPRXIANGGUANDANBAI(CasDANBAI)GONGTONGFAHUIZUOYONG,ERⅡLEIXITONGZHIXUYAOYIZHONGCasDANBAIJIKE,ZHEIWEIQINENGGOUGUANGFANYINGYONGTIGONGLEBIANLITIAOJIAN。

MUQIAN,LAIZIStreptococcus pyogenes DECRISPR-Cas9XITONGYINGYONGZUIWEIGUANGFAN。Cas9 DANBAI(HANYOULIANGGEHESUANMEIJIEGOUYU,KEYIFENBIEQIEGEDNA LIANGTIAODANLIAN。Cas9SHOUXIANYUcrRNAJItracrRNAJIEHECHENGFUHEWU,RANHOUTONGGUOPAMXULIEJIEHEBINGQINRUDNA,XINGCHENGRNA-DNAFUHEJIEGOU,JINERDUIMUDEDNASHUANGLIANJINXINGQIEGE,SHIDNASHUANGLIANDUANLIE。 

YOUYUPAMXULIEJIEGOUJIANDAN(5’-NGG-3’),JIHUKEYIZAISUOYOUDEJIYINZHONGZHAO DAODALIANGBADIAN,YINCIDEDAOGUANGFANDEYINGYONG。CRISPR-Cas9XITONGYIJINGCHENGGONGYINGYONGYUZHIWU、XIJUN、JIAOMU、YULEIJIBURUDONGWUXIBAO,SHIMUQIANZUIGAOXIAODEJIYINZUBIANJIXITONG。 

TONGGUOJIYINGONGCHENGSHOUDUANDUIcrRNAHEtracrRNAJINXINGGAIZAO,JIANGQILIANJIEZAIYIQIDEDAOsgRNA(single guide RNA)。RONGHEDERNAJUYOUYUYESHENGXINGRNALEISIDEHUOLI,DANYINWEIJIEGOUDEDAOLEJIANHUAGENGFANGBIANYANJIUZHESHIYONG。TONGGUOJIANGBIAODAsgRNADEYUANJIANYUBIAODACas9DEYUANJIANXIANGLIANJIE,DEDAOKEYITONGSHIBIAODALIANGZHEDEZHILI,JIANGQIZHUANRANXIBAO,BIANNENGGOUDUIMUDEJIYINJINXINGCAOZUO。

 

 

 

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