汉恒生物CRISPR/Cas9 亚博app官网及服务

●　DUOZHONGBIAOQIANDECRISPR/Cas9 ZHILI

●　CRISPR/Cas9MANBINGDU、XIANBINGDUJIXIANXIANGGUANBINGDU（AAV）BAOZHUANG

●　CRISPR/Cas9DANKELONGQIAOCHUXIBAOZHU

CRISPR/Cas9 基因敲除原理介绍

CRISPR (clustered, regularly interspaced, short palindromic repeats)SHIYIZHONGLAIZIXIJUNJIANGJIERUQINDEBINGDU DNA HUOQITAWAIYUAN DNA DEMIANYIJIZHI。ZAIXIJUNJIGUXIJUNZHONG，CRISPRXITONGGONGFENCHENG3LEI，QIZHONGⅠLEIHEⅢLEIXUYAODUOZHONGCRISPRXIANGGUANDANBAI（CasDANBAI）GONGTONGFAHUIZUOYONG，ERⅡLEIXITONGZHIXUYAOYIZHONGCasDANBAIJIKE，ZHEIWEIQINENGGOUGUANGFANYINGYONGTIGONGLEBIANLITIAOJIAN。

MUQIAN，LAIZIStreptococcus pyogenes DECRISPR-Cas9XITONGYINGYONGZUIWEIGUANGFAN。Cas9 DANBAI（HANYOULIANGGEHESUANMEIJIEGOUYU，KEYIFENBIEQIEGEDNA LIANGTIAODANLIAN。Cas9SHOUXIANYUcrRNAJItracrRNAJIEHECHENGFUHEWU，RANHOUTONGGUOPAMXULIEJIEHEBINGQINRUDNA，XINGCHENGRNA-DNAFUHEJIEGOU，JINERDUIMUDEDNASHUANGLIANJINXINGQIEGE，SHIDNASHUANGLIANDUANLIE。 

YOUYUPAMXULIEJIEGOUJIANDAN（5’-NGG-3’），JIHUKEYIZAISUOYOUDEJIYINZHONGZHAO DAODALIANGBADIAN，YINCIDEDAOGUANGFANDEYINGYONG。CRISPR-Cas9XITONGYIJINGCHENGGONGYINGYONGYUZHIWU、XIJUN、JIAOMU、YULEIJIBURUDONGWUXIBAO，SHIMUQIANZUIGAOXIAODEJIYINZUBIANJIXITONG。 

TONGGUOJIYINGONGCHENGSHOUDUANDUIcrRNAHEtracrRNAJINXINGGAIZAO，JIANGQILIANJIEZAIYIQIDEDAOsgRNA（single guide RNA）。RONGHEDERNAJUYOUYUYESHENGXINGRNALEISIDEHUOLI，DANYINWEIJIEGOUDEDAOLEJIANHUAGENGFANGBIANYANJIUZHESHIYONG。TONGGUOJIANGBIAODAsgRNADEYUANJIANYUBIAODACas9DEYUANJIANXIANGLIANJIE，DEDAOKEYITONGSHIBIAODALIANGZHEDEZHILI，JIANGQIZHUANRANXIBAO，BIANNENGGOUDUIMUDEJIYINJINXINGCAOZUO。

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