服务简介

       

Cas9DANBAIDECDSZHANGDA4kb，KELONGNANDUHEBAODUNANDUDOUXIANGDUIDA，JIANGCas9JIYINGAOXIAODAORUXIBAOSHIYINGYONGCas9/CRISPRXITONGJINXINGJIYINQIAOCHUDENANDIANZHIYI，JIDADIXIANZHIKEGONGXUANZEDEJIANGJIYINDAORUXIBAODEFANGFA。BINGQIEZAIYONGCRISPR/Cas9XITONGJINXINGJIYINQIAOCHUSHI，HAIXUYAOTONGSHIZHUANRUSHIBIEBADIANDEgRNA，SHAIXUANYANGXINGXIBAODEKANGXINGJIYINHUOYINGGUANGJIYIN，YONGTONGYUANZHONGZUFAJINXINGJIYINQIAOCHU，HAIXUYAODAORUTONGYUANZHONGZUMOBAN。RUCIDUODEJIYINGONGTONGBIAODA，HENNANDEDAOJIAOGAODEJIYINBIANJIXIAOLV，ZAOCHENGHOUXUDEYANGXINGKELONGSHAIXUANHEJIANCEGONGZUONANDUDA。

HANHENGSHENGWUDUCHUANGXINGYANFACHUMANBINGDUCas9BIAODATIXI，GAITIXICAIYONGMANBINGDUTIXIXIANZAIXIBAOZHONGWENDINGBIAODACas9DANBAI，GOUJIANWENDINGBIAODACas9DANBAIDEXIBAOXI，ZAIZAIGAIXIBAOXIDEJICHUSHANGDAORUgRNAHEJIYINQIAOCHUSHIYANZHONGYONGDAODEQITAYUANJIANYONGYUTEYIXINGJIYINQIAOCHU。

 

 

 亚博app官网优势

 

a、SHIYANFANGANSHEJIGENGLINGHUO。YOUYUBUBIBIAODADADANBAICas9，KEYIGENJUXIBAOTEXINGXUANZEHUAXUEZHUANRAN、XIANBINGDU、XIANXIANGGUANBINGDUDENGDUOZHONGFANGFADAORUHUOQIAOCHUXIANGGUANJIYIN；

 

b、TIGAOJIYINQIAOCHUXIAOLV。ZAIWENDINGBIAODACas9DANBAIDEXIBAOXISHANGJINXINGJIYINQIAOCHUBISHUNSHIZHUANRANCas9JINXINGJIYINQIAOCHUDEXIAOLVGENGGAO；

 

c、KEDUITONGYIXIBAOJINXINGDUOGEJIYINQIAOCHUSHIYAN。DUIYUXUYAOZAITONGYIGEXIBAOXIZHONGJINXINGDUOGEJIYINDEBIANJIHUOXUYAOZHANGQIYONGYIGEXIBAOMOXINGJINXINGDUOXIANGJIYINYANJIU，XIANGOUJIANYIGECas9DANBAIWENDINGBIAODADEXIBAOXIKEXIANZHUTIGAOHOUXUSHIYANDEXIAOLV，JIANGDISHIYANNANDU。

 

 

服务特点

 

a、SHIYONGYUZAITONGYIGEXIBAOXIZHONGXUYAOJINXINGDUOGEJIYINQIAOCHUDESHIYAN；

b、JIYINQIAOCHUXIAOLVBIZHIJIEZHILIZHUANRANGENGGAO；

c、TIGONGDUOZHONGBUTONGYINGGUANGHEKANGXINGBIAOJIDEMANBINGDUBIAODAZAITI，GENGYISHAIXUANDAOJIYINQIAOCHUCHENGGONGXIBAO；

d、JIESHOUcas9DANBAIWENDINGBIAODABUTONGXIBAOXIDINGZHI。

 

 

服务流程

 

 

 

A、cas9ZAITIGOUJIANJIMANBINGDUBAOZHUANG

JIANGcas9JIYINCDSQUKELONGZHIMANBINGDUZAITI，BINGJINXINGcas9MANBINGDUBAOZHUANG。

B、WENDINGZHUSHAIXUAN

JIANGcas9MANBINGDUGANRANTEDINGDEMUDEXIBAO，JINXINGcas9WENDINGBIAODADEXIBAOZHUSHAIXUAN。

 

 

CANKAOWENXIAN

   1、MicroRNA-30 family members regulate calcium/calcineurin signaling in podocytes；

     （2016年发表于JCI，作者：刘志红）

   2、TGF-β1 promotes osteosarcoma cell migration and invasion through the miR-143-versican pathway；

     （2014年发表于Cellular Physiology and Biochemistry，作者：Shaohua Li and Tao Cheng）

   3、miR-451a Inhibited Cell Proliferation and Enhanced Tamoxifen Sensitive in Breast Cancer via Macrophage Migration Inhibitory Factor；

     （2015年发表于BioMed Research International，作者：李红）

   4、Smad4 mediated BMP2 signal is essential for the regulation of GATA4 and Nkx2. 5 by affecting the histone H3 acetylation in H9c2 cells；

     （2014年发表于Biochemical and Biophysical Research Communications，作者：田杰）

   5、Effects and mechanism of downregulation of COX‑2 expression by RNA interference on proliferation and apoptosis of human breast cancer MCF‑7 cells；

     （2014年发表于Molecular Medicine Reports，作者：hui Han）

   6、MicroRNA-486-5p targeting PIM-1 suppresses cell proliferation in breast cancer cells；

     （2014年发表于Tumor Biology，作者：Zhenlin Yang）

   7、Cyc1 silencing sensitizes osteosarcoma cells to trail-induced apoptosis；

     （2014年发表于Cell Physiol Biochem，作者：Ke Zhou, and Biao Cheng）

   8、MicroRNA-23a is involved in tumor necrosis factor-α induced apoptosis in mesenchymal stem cells and myocardial infarction；

     （2014年发表于Experimental and Molecular Pathology，作者：Yu Zhuang）

   9、Role of human pulmonary fibroblast-derived MCP-1 in cell activation and migration in experimental silicosis；

     （2014年发表于Toxicology and Applied Pharmacology）

   10、CXCR4-targeted near-infrared imaging allows detection of orthotopic and metastatic human osteosarcoma in a mouse model；

     （2015年发表于Scientific Reports，作者：周勇）

   11、Role of high-mobility group box 1 in methamphetamine-induced activation and migration of astrocytes；

     （2015年发表于Journal of Neuroinflammation，作者：姚红红）

   12、The role of MCPIP1 in ischemia/reperfusion injury-induced HUVEC migration and apoptosis；

     （2015年发表于Cell Physiol Biochem，作者：Jie Chao）

   13、P50-associated COX-2 extragenic RNA (PACER) overexpression promotes proliferation and metastasis of osteosarcoma cells by activating COX-2 gene；

     （2015年发表于Tumor Biology，作者：Jianru Xiao）

   14、CRCT1 regulated by microRNA-520 g inhibits proliferation and induces apoptosis in esophageal squamous cell cancer；

     （2015年发表于Tumor Biology，作者：Zhiming Chen）

   15、Overexpression of Hiwi Inhibits the Growth and Migration of Chronic Myeloid Leukemia Cells；

     （2015年发表于Cell Biochemistry and Biophysics，作者：Ziling Liu）

   16、RNF43 inhibits cancer cell proliferation and could be a potential prognostic factor for human gastric carcinoma；

     （2015年发表于Cell Physiol Biochem，作者：Lin Chen）

   17、ZC3H12D attenuated inflammation responses by reducing mRNA stability of proinflammatory genes；

     （2015年发表于Molecular Immunology，作者：Tao Jianga）

   18、MiR-146b-5p promotes metastasis and induces epithelial-mesenchymal transition in thyroid cancer by targeting ZNRF3；

     （2015年发表于Cell Physiol Biochem，作者：Xiaoping Zhang and Youben Fan）

   19、HSF1 Relieves Amyloid-β-Induced Cardiomyocytes Apoptosis；

     （2015年发表于Cell Biochemistry and Biophysics，作者：Beiru Zhang）

   20、Interaction between TNFR1 and TNFR2 dominates the clinicopathologic features of human hypopharyneal carcinoma；

     （2015年发表于Tumor Biology，作者：Qi Song）

   21、Periostin mediates TGF-β-Induced epithelial mesenchymal Transition in prostate cancer cells；

     （2015年发表于Cell Physiol Biochem，作者：Chuanyu Sun and Guowei Xia）

   22、CD49f acts as an inflammation sensor to regulate differentiation, adhesion, and migration of human mesenchymal stem cells；

     （2015年发表于STEM CELLS，作者：Ran Xiao）

   23、MCPIP1 regulates alveolar macrophage apoptosis and pulmonary fibroblast activation after in vitro exposure to silica；

     （2015年发表于Journal of Neuroinflammation ，作者：姚红红）

   24、MicroRNA-33b inhibits tumor cell growth and is associated with prognosis in colorectal cancer patients；

     （2015年发表于Clinical and Translational Oncology，作者：孙荣勋）

   25、MCPIP1 regulates alveolar macrophage apoptosis and pulmonary fibroblast activation after in vitro exposure to silica；

     （2015年发表于ToxSci Advance Access，作者：Jie Chao）

   26、miR‐152 induces human dental pulp stem cell senescence by inhibiting SIRT7 expression；

     （2015年发表于FEBS letters，作者：Jingping Liang）

   27、Lentivirus-mediated PHLDA2 overexpression inhibits trophoblast proliferation, migration and invasion, and induces apoptosis；

     （2015年发表于International Journal of Molecular Medicine，作者：Hui Li）

   28、miR-486-5p inhibits cell growth of papillary thyroid carcinoma by targeting fibrillin-1；

     （2015年发表于Biomedicine & Pharmacotherapy，作者：Jianqing Tian ）

   29、Over-expression of miR-451a can enhance the sensitivity of breast cancer cells to tamoxifen by regulating 14-3-3ζ, estrogen receptor α, and autophagy；

     （2016年发表于Life Sciences，作者：Li-Mimg Zhou、Yuan-Yuan Zhang ）

   30、MicroRNA-30a promotes chondrogenic differentiation of mesenchymal stem cells through inhibiting Delta-like 4 expression；

     （2016年发表于Life Sciences，作者：Ye Tian）

   31、MicroRNA-33 promotes the replicative senescence of mouse embryonic fibroblasts by suppressing CDK6；

     （2016年发表于Biochemical and Biophysical Research Communications，作者：Xinguang Liu ）

   32、Silencing of Paternally Expressed Gene 10 Inhibits Trophoblast Proliferation and Invasion；

     （2015年发表于PloS one，作者：Tao Meng）

   33、Repair of critical-sized bone defects with anti-miR-31-expressing bone marrow stromal stem cells and poly (glycerol sebacate) scaffolds；

     （2014年发表于European cells & materials，作者：Deng Yuan）

   34、MicroRNA-22 induces endothelial progenitor cell senescence by targeting AKT3；

     （2014年发表于Cell Physiol Biochem ，作者：Xu Z）

   35、A lentiviral sponge for miRNA-21 diminishes aerobic glycolysis in bladder cancer T24 cells via the PTEN/PI3K/AKT/mTOR axis；

     （2015年发表于Tumor Biology，作者：Changjun Yin）

   36、p53/PUMA expression in human pulmonary fibroblasts mediates cell activation and migration in silicosis；

     （2015年发表于Sci Rep，作者：Jie Chao）

   37、Endocytosis mediated by Caveolin-1 inhibits activity of matrix metalloproteinase-2 in human renal proximal tubular cells under hypoxia；

     （2016年发表于Int J Clin Exp Pathol，作者： Jun Dou ）

   38、MTSS1 suppresses cell migration and invasion by targeting CTTN in glioblastoma；

     （2015年发表于Journal of Neuro-Oncology，作者：Qigui Qi）

   39、PFDN1, an indicator for colorectal cancer prognosis, enhances tumor cell proliferation and motility through cytoskeletal reorganization；

     （2015年发表于Medical Oncology，作者：Minhua Zheng ）

   40、Human Dental Pulp Stem Cells via the NF-κB Pathway；

     （2015年发表于Cell Physiol Biochem，作者：Jingping Liang ）

   41、Clinical and therapeutic significance of sirtuin-4 expression in colorectal cancer；

     （2016年发表于Oncol Rep，作者：Peng Z）

   42、58-F, a flavanone from Ophiopogon japonicus, prevents hepatocyte death by decreasing lysosomal membrane permeability；

     （2016年发表于Sci Rep，作者：Xiaoling Wang）

   43、Study of Arsenic Sulfide in Solid Tumor Cells Reveals Regulation of Nuclear Factors of Activated T-cells by PML and p53；

     （发表于Sci Rep，作者：Chen S1）