自噬整体研究服务

 

 

 

自噬作为时下的研究热点,有非常深远的研究意义。汉恒生物经验丰富的自噬研究团队,可以为您提供自噬研究的整体服务,省时,节力,专业。

 

 

一、自噬病毒工具

 

HANHENGSHENGWUYOUDUOZHONGZISHIBINGDUYANJIUGONGJUKEYITIGONG,BIANYUZHIJIEGANRANMUDEXIBAOHOUZHIGUANDIGUANCHAXIBAOZISHIDEZHENGTISHUIPING;

 

1)GFP-LC3单荧光自噬指示病毒系统

 

 GFP-LC3BINGDUXITONGKEGAOXIAOGANRANMUDEXIBAO,BIAODAGFP-LC3,GANRANGANRANHOUXIBAOKEZAIYINGGUANGXIANWEIJINGXIASHISHIGUANCHAZISHIDEZHENGTISHUIPING;

 

YOUYUDIANJINGHAOSHIZHANG,BULIYUJIANCE(Monitoring)ZISHIXINGCHENG。WOMENLIYONGLC3ZAIZISHIXINGCHENGGUOCHENGZHONGFASHENGJUJIDEXIANXIANGKAIFACHULEGFP-LC3ZHISHIJISHU:WUZISHISHI,GFP-LC3RONGHEDANBAIMISANZAIBAOJIANGZHONG;ZISHIXINGCHENGSHI,GFP-LC3RONGHEDANBAIZHUANWEIZHIZISHITIMO,ZAIYINGGUANGXIANWEIJINGXIAXINGCHENGDUOGEMINGLIANGDELVSEYINGGUANGBANDIAN,YIGEBANDIANXIANGDANGYUYIGEZISHITI,KEYITONGGUOJISHULAIPINGJIAZISHIHUOXINGDEGAODI。HANHENGSHENGWUYIKAIFACHUGAOXIAODEPINGJIAYONGGFP-LC3BINGDUZAITI,TONGGUOSHUNSHIGAOXIAOGANRANXIBAO,PEIHEHUOXIBAOGONGZUOZHANCHENGGONGPINGJIAZISHILIU。

 

2)mRFP-GFP-LC3双荧光自噬指示病毒系统

 

HANHENGSHENGWUKEJI(SHANGHAI)YOUXIANYABOappGUANWANGYIKAIFACHUYONGYUBIAODAmRFP-GFP-LC3 RONGHEDANBAIDEBINGDUYABOappGUANWANG。mRFP YONGYUBIAOJIJIZHUIZONGLC3,GFP DEJIANRUOKEZHISHIRONGMEITIYUZISHIXIAOTIDERONGHEXINGCHENGZISHIRONGMEITI,JIYOUYUGFP YINGGUANGDANBAIDUISUANXINGMINGAN,DANGZISHITIYURONGMEITIRONGHEHOUGFP YINGGUANGFASHENGCUIMIE,CISHIZHINENGJIANCEDAOHONGSEYINGGUANG。 YIDUOZHONGSHUANGYINGGUANGBINGDUXITONGTIGONG,JUTIRUXIA:

 

       a)mRFP-GFP-LC3腺病毒系统——可高效感染目的细胞,表达mRFP-GFP-LC3,感染后细胞可在荧光显微镜下实时观察自噬发生过程;

 

       b)mRFP-GFP-LC3慢病毒系统——可以稳定表达持续检测细胞的自噬流检测;

 

      c)mRFP-GFP-LC3腺相关病毒(AAV)系统——最有效的在体自噬流检测工具;

 

 

除此之外,我们还提供参与自噬的常见基因的病毒现货Beclin1、Atg7等满足您实验的需求,您可以点击查询您要研究的基因是否有现货——病毒现货查询系统

 

 

二、自噬检测以及整体服务

 

1)自噬检测服务

 

CHANGGUIZISHIJIANCEFUWUBAOKUOwestern blotJIANCEXIBAOZISHIDESHUIPING,YOUHUITIGONGZISHICHANGGUIKANGTILC3、p62YIJIbeclin1DENG,WOMENDEYANJIUTUANDUIZHANGQIWEIKEHUTIGONGZISHIJISHUFUWU,YOUFENGFUDEZISHIYANJIUJINGYAN,WOMENDEYOUSHISHIZHOUQIDUAN,ZHILIANGGAO;

 

       a)LIYONGWestern BlotJIANCELC3-II/IBIZHIDEBIANHUAYIPINGJIAZISHIXINGCHENG。ZISHIXINGCHENGSHI,BAOJIANGXINGLC3(JILC3-I)HUIMEIJIEDIAOYIXIAODUANDUOTAI,ZHUANBIANWEI(ZISHITI)MOXING(JILC3-II),YINCI,LC3-II/IBIZHIDEDAXIAOKEGUJIZISHISHUIPINGDEGAODI。(Note:LC3KANGTIDUILC3-IIYOUGENGGAODEQINHELI,HUIZAOCHENGJIAYANGXING。FANGFA2HE3XUJIEHESHIYONG,TONGSHIXUKAOLVRONGMEITIHUOXINGDEYINGXIANG。)

 

       b)LIYONGWestern BlotJIANCEp62DANBAILAIPINGJIAZISHIYIJIZISHILIUDEQIANGRUO:QICHUZISHISUOJIANGJIEDEDIWUBEIRENWEISHISUIJIDE,DANSHIHOULAIDEYANJIUBIAOMINGYOUXIEDANBAISHIseletivelyJIANGJIEDE,ZAIZHEIXIEDANBAIZHIZHONGYANJIUDEZUIWEITOUCHEDESHIDANBAIp62, p62DANBAISHUIPINGDEDUOSHAOYUZISHILIUDEQIANGRUOYOUZHEFANBILIGUANXI)。

 

       c)HANHENGSHENGWUKETIGONGQITAZISHIXIANGGUANDANBAIDEJIANCEFUWU,XIANGQINGQINGZIXUNHANHENGJISHUGONGCHENGSHI,MIANFEIZIXUNREXIAN:400-092-0065!

 

2)LC3病毒感染服务

 

FANGOUMAIBENYABOappGUANWANGZISHIBINGDUYANJIUGONGJU,WOMENCHENGNUOMIANFEIZHIDAOXIBAOBINGDUGANRANSHIYAN,YIQUEBAONINDESHIYANXIAOGUOJIANQUANXING,CIWAIWOMENHAIHUITIGONGXIANBINGDUSHIYONGXIANGGUANZILIAOJIWENXIANGONGNINCANKAO;

 

3)自噬电镜服务

 

DIANJINGZUOWEIZISHIJIANCEDEJINZHIBIAO,YIZHISHIGAOZHILIANGKEYANLUNWENBUKEHUOQUEDESHUJU。KEHUTIGONGYANGPIN,WOMENWEIQIJIANCEXIBAOHUOZHEZUZHIDEZISHIYIJIZISHILIUDESHUIPING,BINGQIEFUZHUKEHUJINXINGSHUJUFENXIZHENGLI,XIANGXUNHANHENGZISHIGONGCHENGSHI;

YOUYUZISHITISHUYUYAXIBAOJIEGOU,PUTONGGUANGJINGXIAGUANCHABUDAOZISHITIDEXINGCHENG,YINCI,ZHIJIEGUANCHAZISHITIXUZAITOUSHEDIANJINGXIA。PhagophoreDETEZHENGWEI:XINYUEZHUANGHUOBEIZHUANG,SHUANGCENGHUODUOCENGMO,YOUBAORAOBAOJIANGCHENGFENDEQUSHI。ZISHITI(AV1)DETEZHENGWEI:SHUANGCENGHUODUOCENGMODEYEPAOZHUANGJIEGOU,NEIHANBAOJIANGCHENGFEN,RUXIANLITI、NEIZHIWANG、HETANGTIDENG。ZISHIRONGMEITI(AV2)DETEZHENGWEI:DANCENGMO,BAOJIANGCHENGFENYIJIANGJIE。(autophagic vacuole,AV)

 

4)汉恒生物可提供自噬研究整体科研服务

 

HANHENGSHENGWUYOUZHANGQIGUANZHUZISHILINGYUDEJISHUZHICHIYIJISHIYANCAOZUOYUAN,WOMENKEYIWEIKEHUTIGONGCONGKETISHEJIDAOSHIYANCAOZUOBAOKUOHOUXUSHIYANJIEGUODEZHENGLIDENGFUWU。JUTIQINGLIANXIHANHENGDEKEFUDAIBIAO,WOMENDEJISHUGONGCHENGSHIJIANGYUNINZUOJINYIBUXIANGJINDIGOUTONG。MIANFEIZIXUNREXIAN:400-092-0065

 
 

三、自噬研究相关试剂

 

HANHENGSHENGWUKEYIGENJUNINDESHIYANXUQIUWEININTIGONGZUISHIYONGDESHIJIYABOappGUANWANG,RANGNINYONGDEFANGXIN,SHENGXIN!


 

 

部分客户发表文献节选:

 

● Fusobacterium nucleatum Promotes Chemoresistance to Colorectal Cancer by Modulating Autophagy

   (ZAZHI:CELL,IF=36.216,SHANGHAIJIAOTONGDAXUEFUSHURENJIYIYUAN)

● Melatonin ameliorates hypoglycemic stress-induced brain endothelial tight junction injury by inhibiting protein nitration of TP53-induced glycolysis and apoptosis regulator

   (ZAZHI:journal of pineal research,IF=15.211,ZHEJIANGDAXUE)

● The immunoproteasome catalytic ?5i subunit regulates cardiac hypertrophy by targeting the autophagy protein ATG5 for degradation

   (ZAZHI:SCIENCE ADVANCES,IF=12.804,DALIANYIKEDAXUEFUSHUDIYIYIYUAN)

● Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling

   (ZAZHI:Nature Communications,IF=11.878,SHANDONGDAXUE)

● TRB3 links insulin/IGF to tumour promotion by interacting with p62 and impeding autophagic/proteasomal degradations

   (ZAZHI:Nature Communications,IF=11.878,BEIJINGXIEHEYIYUAN)

● Endoplasmic reticulum stress induced by tunicamycin and thapsigargin protects against transient ischemic brain injury: Involvement of PARK2-dependent mitophagy

   (ZAZHI:Autophagy,IF=11.059,ZHEJIANGDAXUE)

● PSMD10/gankyrin induces autophagy to promote tumor progression through cytoplasmic interaction with ATG7 and nuclear transactivation of ATG7 expression

   (ZAZHI:Autophagy,IF=11.059,DIERJUNYIDAXUEFUSHUDONGFANGGANDANYIYUAN)

● Mir143-BBC3 cascade reduces microglial survival via interplay between apoptosis and autophagy: Implications for methamphetamine-mediated neurotoxicity

   (ZAZHI:Autophagy,IF=11.059,DONGNANDAXUE)

● BNIP3L/NIX-mediated mitophagy protects against ischemic brain injury independent of PARK2

   (ZAZHI:Autophagy,IF=11.059,ZHEJIANGDAXUE)

● Ai-lncRNA EGOT enhancing autophagy sensitizes paclitaxel cytotoxicity via upregulation of ITPR1 expression by RNARNA and RNA-protein interactions in human cancer

   (ZAZHI:molecular cancer,IF=10.679,HAERBINYIKEDAXUE)

● Iron oxide nanoparticles promote macrophage autophagy and inflammatory response through activation of toll-like Receptor-4 signaling

   (ZAZHI:Biomaterials,IF=10.273,SICHUANDAXUE)

● The lncRNA HOTAIRM1 regulates the degradation of PML-RARA oncoprotein and myeloid cell differentiation by enhancing the autophagy pathway

   (ZAZHI:Cell Death and Differentiation,IF=8.339,ZHONGSHANDAXUE)

● PrLZ increases prostate cancer docetaxel resistance by inhibiting LKB1/AMPK-mediated autophagy

   (ZAZHI:Theranostics,IF=8.063,XIANJIAOTONGDAXUE)

 

 

 

 

 

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