自噬作为时下的研究热点，有非常深远的研究意义。汉恒生物经验丰富的自噬研究团队，可以为您提供自噬研究的整体服务，省时，节力，专业。

 

 

一、自噬病毒工具

 

HANHENGSHENGWUYOUDUOZHONGZISHIBINGDUYANJIUGONGJUKEYITIGONG，BIANYUZHIJIEGANRANMUDEXIBAOHOUZHIGUANDIGUANCHAXIBAOZISHIDEZHENGTISHUIPING；

 

1）GFP-LC3单荧光自噬指示病毒系统

 

 GFP-LC3BINGDUXITONGKEGAOXIAOGANRANMUDEXIBAO，BIAODAGFP-LC3，GANRANGANRANHOUXIBAOKEZAIYINGGUANGXIANWEIJINGXIASHISHIGUANCHAZISHIDEZHENGTISHUIPING；

 

YOUYUDIANJINGHAOSHIZHANG，BULIYUJIANCE（Monitoring）ZISHIXINGCHENG。WOMENLIYONGLC3ZAIZISHIXINGCHENGGUOCHENGZHONGFASHENGJUJIDEXIANXIANGKAIFACHULEGFP-LC3ZHISHIJISHU：WUZISHISHI，GFP-LC3RONGHEDANBAIMISANZAIBAOJIANGZHONG；ZISHIXINGCHENGSHI，GFP-LC3RONGHEDANBAIZHUANWEIZHIZISHITIMO，ZAIYINGGUANGXIANWEIJINGXIAXINGCHENGDUOGEMINGLIANGDELVSEYINGGUANGBANDIAN，YIGEBANDIANXIANGDANGYUYIGEZISHITI，KEYITONGGUOJISHULAIPINGJIAZISHIHUOXINGDEGAODI。HANHENGSHENGWUYIKAIFACHUGAOXIAODEPINGJIAYONGGFP-LC3BINGDUZAITI，TONGGUOSHUNSHIGAOXIAOGANRANXIBAO，PEIHEHUOXIBAOGONGZUOZHANCHENGGONGPINGJIAZISHILIU。

 

2）mRFP-GFP-LC3双荧光自噬指示病毒系统

 

HANHENGSHENGWUKEJI（SHANGHAI）YOUXIANYABOappGUANWANGYIKAIFACHUYONGYUBIAODAmRFP-GFP-LC3 RONGHEDANBAIDEBINGDUYABOappGUANWANG。mRFP YONGYUBIAOJIJIZHUIZONGLC3，GFP DEJIANRUOKEZHISHIRONGMEITIYUZISHIXIAOTIDERONGHEXINGCHENGZISHIRONGMEITI，JIYOUYUGFP YINGGUANGDANBAIDUISUANXINGMINGAN，DANGZISHITIYURONGMEITIRONGHEHOUGFP YINGGUANGFASHENGCUIMIE,CISHIZHINENGJIANCEDAOHONGSEYINGGUANG。 YIDUOZHONGSHUANGYINGGUANGBINGDUXITONGTIGONG，JUTIRUXIA：

 

       a）mRFP-GFP-LC3腺病毒系统——可高效感染目的细胞，表达mRFP-GFP-LC3，感染后细胞可在荧光显微镜下实时观察自噬发生过程；

 

       b）mRFP-GFP-LC3慢病毒系统——可以稳定表达持续检测细胞的自噬流检测；

 

      c）mRFP-GFP-LC3腺相关病毒（AAV）系统——最有效的在体自噬流检测工具；

 

 

除此之外，我们还提供参与自噬的常见基因的病毒现货Beclin1、Atg7等满足您实验的需求，您可以点击查询您要研究的基因是否有现货——病毒现货查询系统。

 

 

二、自噬检测以及整体服务

 

1）自噬检测服务

 

CHANGGUIZISHIJIANCEFUWUBAOKUOwestern blotJIANCEXIBAOZISHIDESHUIPING，YOUHUITIGONGZISHICHANGGUIKANGTILC3、p62YIJIbeclin1DENG，WOMENDEYANJIUTUANDUIZHANGQIWEIKEHUTIGONGZISHIJISHUFUWU，YOUFENGFUDEZISHIYANJIUJINGYAN，WOMENDEYOUSHISHIZHOUQIDUAN，ZHILIANGGAO；

 

       a）LIYONGWestern BlotJIANCELC3-II/IBIZHIDEBIANHUAYIPINGJIAZISHIXINGCHENG。ZISHIXINGCHENGSHI，BAOJIANGXINGLC3（JILC3-I）HUIMEIJIEDIAOYIXIAODUANDUOTAI，ZHUANBIANWEI（ZISHITI）MOXING（JILC3-II），YINCI，LC3-II/IBIZHIDEDAXIAOKEGUJIZISHISHUIPINGDEGAODI。（Note：LC3KANGTIDUILC3-IIYOUGENGGAODEQINHELI，HUIZAOCHENGJIAYANGXING。FANGFA2HE3XUJIEHESHIYONG，TONGSHIXUKAOLVRONGMEITIHUOXINGDEYINGXIANG。）

 

       b）LIYONGWestern BlotJIANCEp62DANBAILAIPINGJIAZISHIYIJIZISHILIUDEQIANGRUO:QICHUZISHISUOJIANGJIEDEDIWUBEIRENWEISHISUIJIDE,DANSHIHOULAIDEYANJIUBIAOMINGYOUXIEDANBAISHIseletivelyJIANGJIEDE,ZAIZHEIXIEDANBAIZHIZHONGYANJIUDEZUIWEITOUCHEDESHIDANBAIp62, p62DANBAISHUIPINGDEDUOSHAOYUZISHILIUDEQIANGRUOYOUZHEFANBILIGUANXI)。

 

       c）HANHENGSHENGWUKETIGONGQITAZISHIXIANGGUANDANBAIDEJIANCEFUWU，XIANGQINGQINGZIXUNHANHENGJISHUGONGCHENGSHI，MIANFEIZIXUNREXIAN：400-092-0065!

 

2）LC3病毒感染服务

 

FANGOUMAIBENYABOappGUANWANGZISHIBINGDUYANJIUGONGJU，WOMENCHENGNUOMIANFEIZHIDAOXIBAOBINGDUGANRANSHIYAN，YIQUEBAONINDESHIYANXIAOGUOJIANQUANXING，CIWAIWOMENHAIHUITIGONGXIANBINGDUSHIYONGXIANGGUANZILIAOJIWENXIANGONGNINCANKAO；

 

3）自噬电镜服务

 

DIANJINGZUOWEIZISHIJIANCEDEJINZHIBIAO，YIZHISHIGAOZHILIANGKEYANLUNWENBUKEHUOQUEDESHUJU。KEHUTIGONGYANGPIN，WOMENWEIQIJIANCEXIBAOHUOZHEZUZHIDEZISHIYIJIZISHILIUDESHUIPING，BINGQIEFUZHUKEHUJINXINGSHUJUFENXIZHENGLI，XIANGXUNHANHENGZISHIGONGCHENGSHI；

YOUYUZISHITISHUYUYAXIBAOJIEGOU，PUTONGGUANGJINGXIAGUANCHABUDAOZISHITIDEXINGCHENG，YINCI，ZHIJIEGUANCHAZISHITIXUZAITOUSHEDIANJINGXIA。PhagophoreDETEZHENGWEI：XINYUEZHUANGHUOBEIZHUANG，SHUANGCENGHUODUOCENGMO，YOUBAORAOBAOJIANGCHENGFENDEQUSHI。ZISHITI（AV1）DETEZHENGWEI：SHUANGCENGHUODUOCENGMODEYEPAOZHUANGJIEGOU，NEIHANBAOJIANGCHENGFEN，RUXIANLITI、NEIZHIWANG、HETANGTIDENG。ZISHIRONGMEITI（AV2）DETEZHENGWEI：DANCENGMO，BAOJIANGCHENGFENYIJIANGJIE。（autophagic vacuole，AV）

 

4）汉恒生物可提供自噬研究整体科研服务

 

HANHENGSHENGWUYOUZHANGQIGUANZHUZISHILINGYUDEJISHUZHICHIYIJISHIYANCAOZUOYUAN，WOMENKEYIWEIKEHUTIGONGCONGKETISHEJIDAOSHIYANCAOZUOBAOKUOHOUXUSHIYANJIEGUODEZHENGLIDENGFUWU。JUTIQINGLIANXIHANHENGDEKEFUDAIBIAO，WOMENDEJISHUGONGCHENGSHIJIANGYUNINZUOJINYIBUXIANGJINDIGOUTONG。MIANFEIZIXUNREXIAN：400-092-0065

 
 

三、自噬研究相关试剂

 

HANHENGSHENGWUKEYIGENJUNINDESHIYANXUQIUWEININTIGONGZUISHIYONGDESHIJIYABOappGUANWANG，RANGNINYONGDEFANGXIN，SHENGXIN!

 

 

部分客户发表文献节选：

 

●　Fusobacterium nucleatum Promotes Chemoresistance to Colorectal Cancer by Modulating Autophagy

   （ZAZHI：CELL，IF=36.216，SHANGHAIJIAOTONGDAXUEFUSHURENJIYIYUAN）

●　Melatonin ameliorates hypoglycemic stress-induced brain endothelial tight junction injury by inhibiting protein nitration of TP53-induced glycolysis and apoptosis regulator

   （ZAZHI：journal of pineal research，IF=15.211，ZHEJIANGDAXUE）

●　The immunoproteasome catalytic ?5i subunit regulates cardiac hypertrophy by targeting the autophagy protein ATG5 for degradation

   （ZAZHI：SCIENCE ADVANCES，IF=12.804，DALIANYIKEDAXUEFUSHUDIYIYIYUAN）

●　Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling

   （ZAZHI：Nature Communications，IF=11.878，SHANDONGDAXUE）

●　TRB3 links insulin/IGF to tumour promotion by interacting with p62 and impeding autophagic/proteasomal degradations

   （ZAZHI：Nature Communications，IF=11.878，BEIJINGXIEHEYIYUAN）

●　Endoplasmic reticulum stress induced by tunicamycin and thapsigargin protects against transient ischemic brain injury: Involvement of PARK2-dependent mitophagy

   （ZAZHI：Autophagy，IF=11.059，ZHEJIANGDAXUE）

●　PSMD10/gankyrin induces autophagy to promote tumor progression through cytoplasmic interaction with ATG7 and nuclear transactivation of ATG7 expression

   （ZAZHI：Autophagy，IF=11.059，DIERJUNYIDAXUEFUSHUDONGFANGGANDANYIYUAN）

●　Mir143-BBC3 cascade reduces microglial survival via interplay between apoptosis and autophagy: Implications for methamphetamine-mediated neurotoxicity

   （ZAZHI：Autophagy，IF=11.059，DONGNANDAXUE）

●　BNIP3L/NIX-mediated mitophagy protects against ischemic brain injury independent of PARK2

   （ZAZHI：Autophagy，IF=11.059，ZHEJIANGDAXUE）

●　Ai-lncRNA EGOT enhancing autophagy sensitizes paclitaxel cytotoxicity via upregulation of ITPR1 expression by RNARNA and RNA-protein interactions in human cancer

   （ZAZHI：molecular cancer，IF=10.679，HAERBINYIKEDAXUE）

●　Iron oxide nanoparticles promote macrophage autophagy and inflammatory response through activation of toll-like Receptor-4 signaling

   （ZAZHI：Biomaterials，IF=10.273，SICHUANDAXUE）

●　The lncRNA HOTAIRM1 regulates the degradation of PML-RARA oncoprotein and myeloid cell differentiation by enhancing the autophagy pathway

   （ZAZHI：Cell Death and Differentiation，IF=8.339，ZHONGSHANDAXUE）

●　PrLZ increases prostate cancer docetaxel resistance by inhibiting LKB1/AMPK-mediated autophagy

   （ZAZHI：Theranostics，IF=8.063，XIANJIAOTONGDAXUE）